Expression of Integrin β6 and HAX-1 Correlates with Aggressive Features and Poor Prognosis in Esophageal Squamous Cell Carcinoma
Expression of Integrin β6 and HAX-1 Correlates with Aggressive Features and Poor Prognosis in Esophageal Squamous Cell Carcinoma
Blog Article
Fanni Li,1 Yukui Shang,2 Feiyu Fan Shop - CFL - Jerseys Shi,3 Lei Zhang,3 Jun Yan,3 Qi Sun,3 Junjun She3 1Department of Talent Highland, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710061, People’s Republic of China; 2Department of Basic Medical Sciences, School of Medicine, Tsinghua University, Beijing 100084, People’s Republic of China; 3Department of General Surgery, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710061, People’s Republic of ChinaCorrespondence: Qi Sun; Junjun SheDepartment of General Surgery, The First Affiliated Hospital of Xi’an Jiaotong University, 277 Yanta West Road, Xi’an, Shaanxi 710061, People’s Republic of ChinaTel/Fax +86 29 85433720Email [email protected]; [email protected]: The development of esophageal squamous cell carcinoma (ESCC) is a complicated process in which cell adhesion and motility, mediated by integrins, are involved through connecting the cytoskeleton to extracellular matrix.Different mechanisms via which integrin β 6 participates in cancer invasion and metastasis have been described by numerous studies; however, the expression and clinical significance of integrin β 6 in ESCC remain unknown.
Methods: To investigate the differential expression of integrin β 6 in ESCC, qPCR and immunohistochemistry assays were performed in 10 paired human samples.A total of 137 ESCC samples were further enrolled to evaluate the expression levels of integrin β 6 and its endocytic trafficking regulator HS1-associated protein X-1 (HAX-1), followed by the evaluation of their correlation with clinicopathological parameters.The overall survival was analyzed using the Kaplan–Meier method, with significant variables further evaluated by multivariate Cox regression analyses.Results: The expression of integrin β 6 was markedly increased in ESCC compared with matched adjacent normal tissues.
Among the ESCC samples, positive expression of integrin β 6 was observed in 41.6% tumors, which was associated with histological differentiation, lymph node metastasis and TNM stage.High expression of HAX-1 was detected in 47.4% tumors, and there was a positive relationship between the expression levels of integrin β 6 and HAX-1.
Furthermore, the expression of integrin Bottle Openers β 6 and HAX-1 were independent unfavorable indicators for prognosis.Patients with positive integrin β 6 and high HAX-1 expression demonstrated worst outcomes.Conclusion: The present findings suggested the predictive value of integrin β 6 and HAX-1 as independent indicators of poor prognosis for patients with ESCC, both of which may contribute to the tumor proliferation and metastasis, leading to ESCC progression.Therefore, combined targeting of integrin β 6 and HAX-1 may provide a potential novel approach for the treatment of ESCC.
Keywords: integrin, HAX-1, esophageal squamous cell carcinoma, prognosis.